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Laboratory Tests |
FMD Laboratory Practice |
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FMD Laboratory Practice |
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Infectious material Always regard field samples submitted for testing as potentially infectious to man. Handle only to make suspensions and aliquots, preferably in a laminar flow hood. If spillage of potentially infectious material occurs, apply liberal quantities of disinfectant solution to the contaminated area. As much of the infectious material as possible should be removed starting from the outer area of contamination. All material used should be placed in a container and autoclaved. Take care not to splash virus suspensions over materials not easily disinfected e.g., micropipettes, ELISA plates. Discard all instrument wrappings, instruments, pestles and mortars etc. into disinfectant solution and soak for at least 1 hour, or place in a suitable container partially filled with water and boil for 15 minutes. All micropipette tips should be discarded immediately after use into either disinfectant (0.2% citric acid, 0.4% FAM) or a container for eventual boiling. Rinse bottles etc. containing field samples and suspensions for storage and centrifugation in disinfectant solution immediately after use or upon removal from a laminar flow hood. Follow by a rinse in water. If persons are contaminated, clothing should be wiped with a cloth or sponge soaked in disinfectant before subsequent washing. Any exposed skin which has been contaminated should be washed with disinfectant or, if this is not possible, eyes, nose and mouth should be washed with soap and water. All washing water should be discarded into disinfectant, and swabs for eyes, nose and mouth should also be sterilised before disposal. Reagents Care is required with all laboratory reagents. In particular: OPD is a suspect CARCINOGEN and GLOVES should be worn when this is handled. Unused OPD should be neutralised with 1M HCl before disposal. ETHIDIUM BROMIDE is potentially a CARCINOGEN and GLOVES should be worn when this is handled. EYE-PROTECTION should be worn when making sodium hydroxide, sulphuric and hydrochloric acid. CONCENTRATED SULPHURIC ACID to be diluted should be added to water; water must not be added to the acid. SODIUM AZIDE IS TOXIC, Appropriate care should be taken in handling and disposal. |
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