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Laboratory Tests |
Virus/Antigen Detection |
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Virus Isolation in Tissue Culture |
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Tissue samples from infected animals may not contain sufficient antigen to give a positive identification of FMD virus in the antigen detection ELISA. Live virus in original tissue samples, however, may be amplified by culture on susceptible monolayer cell sheets to produce antigen-rich supernatant fluids suitable for this test. The presence of FMD virus in culture is determined by the observation of virus-specific cytopathic effect (CPE) after inoculation.
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