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Diagnostic Procedure
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Absorbent towels
Bench centrifuge
Freezer: any type in the range -15°C to -20°C.
Colour key buffer solutions pH 4, 7 and 10
Cryopreservation vials: recommended: polypropylene, flat-bottom screw cap with internal thread and O-ring seal (1-5 ml), and appropriate storage racks
ELISA microtitre plates: 96-well, flat-bottomed. NUNC Immunoplate Maxisorp. (Gibco, cat. no4-39454A) (Substitution may alter diagnostic performance). Plates should be stored between +4º and 15ºC
Small glass vials (volume up to 2 ml)
Freezer
Any type in the range -15ºC / -20ºC to +4ºC
Glassware / Plasticware
A selection of beakers (20-4000 ml), flasks, graduated cylinders (10-2000 ml), graduated pipettes (1-20 ml) with suitable safety bulbs, storage bottles with closures (1-100 ml), dilution tubes (2-4 ml) and suitable racks, wash fluid container with tap (5-10 litre) and tubing for connection of this reservoir to plate washer or, alternatively, wash bottles (250 ml).
Incubator: any type of radiant, warm wall incubator or hot room in the range of +35°C to 39°C
Lids for microtitre plates
Marker pens (water-proof) and Adhesive labels
Orbital shaker
Pestle and mortar: any type with a diameter of approximately 7 cm
pH indicator strips range 5 - 10
pH meter: any type with an accuracy of 0.01 pH units; not essential if pH papers available, but may be of assistance in problem solving.
Photometer: Flow Laboratories, Titertek Multiskan Plus MKII microtitre plate reader (or equivalent) with an interference filter of 492 nm; IBM compatible computer, monitor and printer, ELISA data analysis software (useful but not essential).
Pipettes
Flow Laboratories, Titertek Multichannel (or equivalent) pipettes (8 or 12 channel), variable ranges from 5-50 µl and 50-300 µl, and quality tips. Gilson Pipetman (or equivalent) single channel pipettes, variable ranges from 1-10 µl, 5-40 µl, 40-200 µl and 200-1000 µl, and quality tips.
Reagent troughs
Reagent troughs (reservoirs) suitable for simultaneous, multichannel pipetting of a single reagent, and troughs suitable for simultaneous, multichannel pipetting of eight different reagents.
Refrigerator
Any type in the range +2° and 6°C.
Standard laboratory equipment
Measuring cylinders, 10 ml pipettes, assorted small glass bottles and beakers.
Timer
Any type, preferably countdown-type with an audible alarm.
Vortex mixer
Any type with variable speed.
Washer
Flow Laboratories, Titertek Handiwash microplate washer (or equivalent). Alternatively, plastic wash bottles or a wash fluid container connected to suitable tubing may be used.
Water bath
Any type in the range +35°C-39°C: not essential, but useful for rapid warming or thawing of reagents.
Water purification system
Minimum: glass-distilled or de-ionized water.
Optimum: Millipore, Milli-Q (or equivalent), Type 1, 18 megohm, pyrogen-free water.
Weighing balance
Any type with an accuracy of +/- 0.1 g.
Weighing boats
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