|
|
|
Chemicals
Deoxynucleotide triphosphates, dNTPs
(dATP, dGTP, dCTP, dTTP) (100 mM stocks)
Mineral oil
Acetylated bovine serum albumin (BSA)
Agarose (high gelling temperature electrophoresis grade)
Boric acid
Bromophenol blue
TBE (10x) or TAE (20x) buffer
Chloroform
Diethyl pyrocarbonate (DEPC) - treated water
Dithiothreitol
EDTA (disodium salt)
Ethanol
Ethidium bromide
Ficoll 400 (or sucrose, glycerol)
Glycogen
Magnesium chloride
Molecular weight marker DNAs
Potassium chloride
Primer pairs specific for each pathogen to be identified
Propyl alcohol (propan-2-OL)
RNase-free DEPC-treated water
Sodium hydroxide
Taq polymerase
TRIS base
TRIS-HCl
Trizol (Gibco/BRL)
Xylene cyanol
|
