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Laboratory Tests |
Antibody Detection |
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Neutralisation Test |
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Antibody to FMD virus can be detected in the FMD virus neutralisation
test by the ability of the serum to prevent CPE when mixed with virus
of known titre and added to cultures of susceptible cells. The titre of
the serum is defined as that dilution of serum that is able to neutralise
100 TCID50 of virus in 50% of individual inoculated cell monolayers, i.e.,
the 50% end point titre. (Golding
et al., 1976) Readers may find it easier to read the relevant section
of the OIE Manual to familiarise themselves with the outline of the test
before reading this section, which provides details of the protocol used
at the World Reference Laboratory for FMD.
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