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Antibody Detection
ELISA
Neutralisation Test
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Absorbent towels
Bottles
Small glass or plastic
'Carrier plates'
Any make of 96-well U-bottom plate in polystyrene (single-use disposable) or polypropylene (re-useable)
Colour key buffer solutions pH 4, 7 and 10
Cryopreservation vials
Recommended: polypropylene, flat-bottom screw cap with internal thread and O-ring seal (1-5 ml), and appropriate storage racks.
ELISA microtitre plates
96-well, flat-bottomed. NUNC Immunoplate Maxisorp. (Gibco, cat. no.4-39454A) (substitution may alter diagnostic performances). Plates should be stored between +4ºC and 15ºC.
Glassware / Plasticware
A selection of beakers (20-4000 ml), flasks, graduated cylinders (10-2000 ml), graduated pipettes (1-20 ml) with suitable safety bulbs, storage bottles with closures (1-100 ml), dilution tubes (2-4 ml) and suitable racks, wash fluid container with tap (5-10 litre) and tubing for connection of this reservoir to plate washer or, alternatively, wash bottles (250 ml).
Glass vials
Lids for microtitre plates
Marker pens (water-proof) and adhesive labels
pH indicator strips
pH meter
Any type with an accuracy of 0.01 pH units; not essential if pH papers available, but may be of assistance in problem solving.
Photometer
Flow Laboratories, Titertek Multiskan Plus MKII microtitre plate reader (or equivalent) with an interference filter of 492 nm; IBM compatible computer, monitor and printer, ANELISA 2.0 ELISA software programme or equivalent ELISA analysis software package.
Pipettes
Flow Laboratories, Titertek Multichannel (or equivalent) pipettes, variable ranges from
5-50 ml and 50-300 ml, and quality tips.
Gilson Pipetman (or equivalent) single channel pipettes, variable ranges from 1-10 ml,
5-40 ml, 40-200 ml and 200-1000 ml, and quality tips.
plastic, 1 ml, 10 ml
Reagent troughs
Reagent troughs (reservoirs) suitable for simultaneous, multichannel pipetting of a single reagent.
Refrigerator
Any type in the range +2°C to 6°C.
Standard laboratory equipment
Bijoux, universals, measuring cylinders, 10-ml pipettes, assorted glass bottles and beakers.
Timer
Any type, preferably countdown-type with an audible alarm.
Washer
Flow Laboratories, Titertek Handiwash microplate washer (or equivalent). Alternatively, plastic wash bottles or a wash fluid container connected to suitable tubing may be used.
Water bath
Any type in the range +35°C to 39°C: not essential, but useful for rapid warming or thawing of reagents.
Water purification system
Minimum: glass-distilled or de-ionized water.
Optimum: Millipore, Milli-Q (or equivalent), Type 1, 18 megohm, pyrogen-free water.
Weighing balance
Any type with an accuracy of +/- 0.1 g.
Weighing boats
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