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Chemicals
Calcium chloride dihydrate (CaCl2.2H2O)
Citric acid monohydrate (C6H8O7.H2O)
Disodium hydrogen phosphate anhydrous (Na2HPO4)
Magnesium chloride hexahydrate (MgCl2.6H2O)
Potassium chloride (KCl)
Potassium dihydrogen phosphate anhydrous (KH2PO4)
Sodium azide (NaN3)
Sodium bicarbonate anhydrous (NaHCO3)
Sodium carbonate anhydrous (Na2CO3)
Sodium chloride (NaCl)
Sodium hydroxide (NaOH)
Sulphuric acid (conc) (H2SO4)
Hydrochloric acid (1M) (HCl)
Carbonate/bicarbonate buffer capsules (Sigma C3041)
Orthophenylene diamine dihydrochloride (OPD) or
OPD Tablets 30 mg (Sigma P8412)
Hydrogen peroxide, analytical grade, 30%
Phenol red powder
Skimmed milk powder (Marvel or equivalent)
Tween 20 (polyoxyethylene sorbitan monolaurate)
FAM disinfectant (Evans Vanodine International)
Biological reagents
Coating sera:
Rabbit antiserum against standard FMDV virus
Control sera:
Convalescent bovine sera of appropriate
type: strong positive, weak positive and negative
Conjugate:
Peroxidase conjugated rabbit immunoglobulins
to guinea pig immunoglobulins (P141,
Dako Ltd) pre-blocked with normal rabbit
and bovine sera
Detection sera
'Blocked' guinea pig sera against
appropriate standard FMDV serotype, mixed
with equal volume of bovine serum and
heat inactivated at 56ºC for 30 minutes.
(WRL, Pirbright, UK)
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