Laboratory Tests

Antibody Detection

Related
topics

Liquid Phase Blocking ELISA

Antibody Detection ELISA

Neutralisation Test

Traditional antibody assays, such as the virus neutralisation test, rely on cells as an indicator of viral activity. Cells may not only become contaminated but also vary in sensitivity towards the agents being tested. In addition, a wide range of antibodies are involved in the immune response, not just those that neutralise. Therefore, non-sterile assays that can use either live or inactivated antigens, measure all antibody binding to a virus, and use an enzymic reaction as an indicator, are often preferred.

In the antibody detection ELISA test sera are pre-mixed with standard FMD virus before addition to an ELISA plate coated with anti-FMD antibody. If antibody is present in the test sera this will block the standard virus, which will be unable to bind to the coating antibody on the plate. If there is no virus-specific antibody in the test sera then the standard virus will be available to be trapped on the plate, and this will be detected by a positive colour reaction indicating a negative test result. (Hamblin et al., 1986a, Hamblin et al., 1986b).

Equipment Reagents Preparation Method Results

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