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Hypersensitivity Reactions
in Cattle Following Immunisation
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The non-toxicity test may be done either as part of the in vivo safety test in a target species or in laboratory animals.
In the case of cattle, the tongue of each of three healthy, susceptible cattle is injected with 2 ml of vaccine, followed 4 days later by three times the dose indicated on the label using the prescribed route. The animals are observed for a total period of 10 days. Any local or systemic reaction to vaccination should remain insignificant.
Vaccine may be tested in guinea pigs and mice using smaller volumes by
the subcutaneous route. Post-mortem examination of guinea pigs and mice
is made 12 days after vaccination and there should be no evidence of an
abnormal pathology associated with the vaccine.
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