AVIS - Foot and Mouth Disease


Cycle Sequencing Additional Reagents/Equipment Samples for Sequencing Extraction of Virus Reverse Transcription of Virus RNA Choice of Primers PCR Amplification of Reversed Transcribed RNA Agarose Gel Electrophoresis of PCR Products Purification of PCR Products End-Labelling of Oligonucleotide Primers Cycle Sequencing Electrophoresis of Cycle Sequencing Products	Ammonium persulphate Formamide Isopropanol Mercaptoethanol Molecular weight markers Moloney Murine Leukaemia Virus reverse transcriptase (MMLV) 32P-ATP labelled primer PAGE apparatus Photographic processing facility Polyacrylamide gel Repelcote Slab gel drier T4 polynucleotide kinase TEMED X-ray film