Tools
Diagrams
Principle of Polymerase Chain Reaction (PCR)
Cycle Sequencing Additional Reagents/Equipment Samples for Sequencing Extraction of Virus Reverse Transcription of Virus RNA Choice of Primers PCR Amplification of Reversed Transcribed RNA Agarose Gel Electrophoresis of PCR Products Purification of PCR Products End-Labelling of Oligonucleotide Primers Cycle Sequencing Electrophoresis of Cycle Sequencing Products
Cycle Sequencing
Additional Reagents/Equipment
Samples for Sequencing
Extraction of Virus
Reverse Transcription of Virus RNA
Choice of Primers
PCR Amplification of Reversed Transcribed RNA
Agarose Gel Electrophoresis of PCR Products
Purification of PCR Products
End-Labelling of Oligonucleotide Primers
Electrophoresis of Cycle Sequencing Products
Viruses for examination should be amplified by growth in tissue culture. Alternatively, epithelium from a recently ruptured lesion can be used, in which case a 10% suspension of epithelium should be prepared in PBS.
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